The research team of Professor Zhang Luyong from the New Drug Research and Development Center of our school has made a series of progress in the field of electrochemical biosensing
Electrochemical biosensors provide a new tool for portable and cheap detection of biomolecules in the fields of gene analysis, disease diagnosis and drug evaluation. The Zhang Luyong/Sun Duanping research team of the New Drug Research and Development Center of our school aims at early diagnosis of major diseases and new drug screening methods, and constructs a series of new electrochemical biosensors for ultra-high sensitivity detection of biomarkers. The relevant research results are cited by Guangdong Pharmaceutical University As the first unit and communication unit, recently published in Biosensors and Bioelectronics (Chinese Academy of Sciences, IF: 10.257, 2 articles), ACS Applied Materials & Interfaces (Chinese Academy of Sciences, IF: 8.758), Sensors and Actuators B: Chemical ( Chinese Academy of Sciences First District, IF: 7.1), Electrochimica Acta (Chinese Academy of Sciences Second District, IF: 6.215) and Analytica Chimica Acta (Chinese Academy of Sciences Second District, IF: 5.977), two of which were selected as the cover papers of the current issue.
Troponin (cTnI) is currently a biomarker widely used in clinical detection of heart failure, myocardial infarction and other diseases. cTnI is a regulatory protein specific to myocardial tissue. When the myocardium is damaged, free cTnI first escapes from cells and enters the blood circulation to be detected. With the aggravation of myocardial injury, the serum concentration of cTnI gradually increases. The team constructed an enzyme-free electrochemical aptasensor for highly sensitive detection of cTnI. First, the DNA tetrahedral nanostructure was combined with two nucleic acid aptamers Tro4 and Tro6, which were bound to the surface of the electrode through sulfur-gold bonds as capture probes to regulate the density and orientation of the probes on the surface of the gold electrode and improve the capture efficiency of cTnI. The aptamer-functionalized nanoprobes were then constructed using magnetic metal-organic frameworks with large specific surface area and strong catalytic activity. Finally, a super sandwich sandwich structure was constructed on the electrode surface by layer-by-layer assembly method, and the highly sensitive detection of cTnI was realized by electrochemical signal amplification. This method has good selectivity and reproducibility, the detection range is 0.05-100 ng/mL, and the detection limit is 16 pg/mL, which is consistent with the results of the traditional ELISA method, and has good market promotion and application value. Related research results were published in Biosensors and Bioelectronics, 2019, 134, 49-56 and Biosensors and Bioelectronics, 2019, 142, 111578.
Figure 1 Electrochemical aptasensor for ultrasensitive detection of cTnI
Liquid biopsy technology based on circulating tumor cell detection has the advantages of complete tumor molecular information, less invasiveness, and low cost, and is of great significance in cancer diagnosis and individualized drug treatment. At the invitation of the editor of Analytica Chimica Acta, Dr. Sun Duanping analyzed and discussed in detail the application prospects of electrochemical cell sensors in cancer precision diagnosis, drug guidance and drug development, starting from the conventional method of detecting circulating tumor cells, combined with the previous work of our research team . The relevant results were published in Analytica Chimica Acta, 2019, 1082, 1-17, and were selected as the cover article (Outside Front Cover) and feature article (Feature Article). Elsevier's news network New chemistry research wrote a news entitled "Using cancer to find tumors" for a highlight report. The publication of this review of invited manuscripts marks that the research team of Professor Zhang Luyong of our school has received the recognition of international excellent journals and the attention of international peers in the cutting-edge research work related to electrochemical biosensors.
Figure 2 Outside Front Cover for Analytica Chimica Acta, 2019, 1082, 1-17
Reactive oxygen species (ROS) are important intracellular signaling molecules that play important roles in signal conversion and immune activity during protein synthesis, DNA damage, apoptosis, etc. H2O2 is one of the representatives of active oxygen, which is relatively stable and can penetrate out of cells. Therefore, establishing an accurate, rapid and sensitive method for monitoring the dynamic release process of H2O2 in living cells is of great significance for cell research and drug evaluation. The team prepared an enzyme-free electrochemical biosensor by regulating the different crystal morphologies of the same metal-organic framework material, which was used to monitor the release of H2O2 from living cells in real time, providing a simple and fast new method for the evaluation of anticancer drugs. Relevant research results were published in ACS Applied Materials & Interfaces, 2020, 12, 41960-41968, and were selected as the Supplementary Journal Cover of the current issue. On this basis, the team further found that calcined metal organic framework materials and magnetic metal organic framework materials have excellent peroxidase-mimetic activity in electrochemical detection of H2O2. Compared with traditional biological enzymes, it has the advantages of strong catalytic activity, high stability, and storage resistance. Related research results have been published in Sensors and Actuators B: Chemical, 2020, 311, 127909 and Electrochimica Acta, 2020, 359, 136962.
Figure 3 Supplementary Journal Cover for ACS Applied Materials & Interfaces, 2020, 12, 41960-41968
The above work has been supported by the National Natural Science Foundation of China, the Natural Science Foundation of Guangdong Province, the project of the Guangdong Provincial Department of Education, the Key Laboratory of New Drug Discovery and Drugability Evaluation of General Universities in Guangdong Province, the Guangzhou Key Laboratory of New Drug Screening Model System Construction and Application, and the school strong support.
Links to related papers:
Paper 1: https://doi.org/10.1016/j.bios.2019.03.049
Paper 2: https://doi.org/10.1016/j.bios.2019.111578
Paper 3: https://doi.org/10.1016/j.aca.2019.07.054
Paper 4: https://doi.org/10.1021/acsami.0c11269
Paper 5: https://doi.org/10.1016/j.snb.2020.127909
Paper 6: https://doi.org/10.1016/j.electacta.2020.136962
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